Mitochondrial and STR DNA Analysis by Mass Spectrometry Using the Ibis Biosciences, Inc. Platform - A Technology Transition Workshop

• NCJ Number: 236452
• Date Published: February 2009
• Length: 5 pages
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)

Annotation

The purpose of this Technology Transition Workshop is to provide the student with an introduction to biological mass spectrometry; the Ibis T5000 and T6000 platforms, their applications, and assay protocols; an overview of the T5000 hardware and its maintenance; and the use of the Ibis software for data analysis.

Abstract

The purpose of this Technology Transition Workshop is to provide the student with an introduction to biological mass spectrometry; the Ibis T5000 and T6000 platforms, their applications, and assay protocols; an overview of the T5000 hardware and its maintenance; and the use of the Ibis software for data analysis. Students will also receive instruction on mitochondrial DNA analysis; mitochondrial DNA mixture detection, analysis, and interpretation; statistics of polymorphic STR alleles; and future directions of biological mass spectrometry. Use of the Ibis T5000 Mass Spectrometer Platform will be demonstrated, allowing students to take samples that they amplify through analysis and data interpretation using Ibis mtDNA software.