Case Report: Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique

• NCJ Number: 218820
• Author(s): Kong Boom Lim; Primulapathi Jaya; Baktiar Kassim; Lay Hong Seah; Yoon Hin Lee; Ong Koh Chee
• Date Published: July 2000
• Length: 11 pages
• Sale Source: US Dept of Justice Federal Bureau of Investigation
• Publication Format: Document (Online)
• Publication Type: Report (Technical); Case Study
• Language: English
• Country: United States of America
• Note: From Forensic Science Communications, V 2, N 3, July 2000; downloaded June 12, 2007.

Annotation

This article describes work done by the Department of Chemistry, Malaysia, in identifying 13 severely burned victims of a highway collision that involved a tourist bus and a transport truck.

Abstract

Using reference blood samples from their next of kin, all 13 victims were positively identified by the kinship analysis approach that used the RFLP (single locus chemiluminescent enhanced probe system) technique. DNA was extracted from each liquid blood, bone marrow, and tissue sample by using the phenol/chloroform/isoamyl alcohol extraction after proteinase K digestion. The DNA was digested with restriction enzyme Hae III, and the digested DNA subsequently electrophoresed in a 0.8 percent agarose gel. K562 DNA was used an internal control marker, and adenovirus DNA digested with Kpn1 restriction enzyme was used as a visual marker during electrophoresis. An analytical sizing marker composed of 30 bands with fragment sizes that ranged from 526 to 22,621 base pairs was used to determine the size of the alleles of each individual. DNA analysis using the RFLP technique was performed to detect the Hae III restricted fragment alleles at four genetic loci (D2S44, D4S139, D5S110, and D10S28) using the probes YNH24 (GenePrint Light), PH30 (Aces), LH1 (Aces), and TBQ7 (GenePrint Light), respectively. 3 tables, 6 figures, and 6 references