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Case Study of SNPSTR Efficiency in Paternity Testing with Locus Incompatibility

NCJ Number
Forensic Science International: Genetics Volume: 9 Dated: March 2014 Pages: 72-75
Yi Ye; Haibo Luo; Linchuan Liao; Ji Zhang; Wei Wei; Zheng Wang; Yiping Hou
Date Published
March 2014
4 pages
For a paternity case that showed CSF1PO inconsistency in laboratory testing, the alleged father and the son were analyzed using a new approach developed by this study.
The 5 SNP-CSF1PO haplotypes of the alleged father were TGCCT-11 and TGCTC-13, and the sons were GGTTT-12 and TGCTC-12. The sequencing results confirmed the SNPSTR haplotype result obtained by the proposed method Alignment of the sequences showed that the son lost one core repeat at the CSF1PO locus. The authors selected CSF1PO STR and five closely linked SNPs: rs10077461, rs2228422, rs3733673, and rs3829987 in order to establish the SNPSTR. Its potential use in paternity testing was examined. A total of 152 haplotypes from 76 unrelated individuals were obtained by the nested-AS-PCR, and 60 SNPSTR haplotypes were observed. The discriminatory power of the SNPSTR haplotypes was greater than either the STR or SNP haplotype alone. Its SNP part could be used to distinguish fathers from uncles. When SNPSTR was introduced into the calculation of parentage statistics, the paternity probability increased to 99.998 percent. This indicates that SNPSTR should be considered a useful tool in forensic science. 2 tables and 19 references