Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer

• NCJ Number: 246026
• Journal: Journal of Forensic Sciences Volume: 59 Issue: 4 Dated: July 2014 Pages: 1090-1099
• Author(s): Zoltan Szabolcsi M.Sc.; Balazs Egyed Ph.D.; Petra Zenke Ph.D.; Zsolt Padar Ph.D.; Adrienn Borsy Ph.D.; Viktor Steger Ph.D.; Erzsbet Pasztor Ph.D.; Sandor Csanyi D.Sc.; Zsuzsanna Buzas Ph.D.; Laszlo Orosz D.Sc.
• Date Published: July 2014
• Length: 10 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals.

Abstract

Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5-plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II. LD pairwise tests and cross-species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and FST was 0.034 using AMOVA. The average probability of identity (PIave) was at the value of 2.6736 10-15. This low value for PIave nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio (LR) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations. Abstract published by arrangement with Wiley.