Developmental validation of 15 X chromosomal short tandem repeat markers

• NCJ Number: 310868
• Journal: Forensic Science International Genetics Volume: 4 Issue: 1 Dated: 2013 Pages: e142-e143
• Author(s): Toni M. Diegoli; Adrian Linacre; Michael D. Coble
• Date Published: November 2013
• Grant Number(s): 2011-DN-BX-K401
• Sponsoring Agency: National Institute of Justice (NIJ)
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Country: United States of America

Abstract

The use of X chromosomal short tandem repeat (STR) markers has been greatly increasing in the forensic setting. Using guidelines set forth previously for the validation of autosomal and Y STRs, aspects of the feasibility of routine X chromosomal STR use were evaluated. Two mini-X chromosomal STR multiplexes capable of amplifying 15 total markers were developed and utilized to determine allele nomenclature, allele/genotype frequencies, mutation rates, and linkage between markers. Additionally, a concordance study between these multiplexes and a commercially available kit was performed. Here, the authors present an overview of this extensive developmental validation study.

(Publisher abstract provided.)