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Field Contamination of Skeletonized Human Remains with Exogenous DNA

NCJ Number
243753
Journal
Journal of Forensic Sciences Volume: 58 Issue: 1 Dated: January 2013 Pages: 206-209
Author(s)
Suni M. Edson, M.S.; Alexander F. Christensen, Ph.D.
Date Published
January 2013
Length
4 pages
Annotation
The Armed Forces DNA Identification Laboratory reports the mitochondrial DNA (mtDNA) sequences of over 800 skeletal samples a year for the Joint POW/MIA Accounting Command-Central Identification Laboratory.
Abstract
The Armed Forces DNA Identification Laboratory reports the mitochondrial DNA (mtDNA) sequences of over 800 skeletal samples a year for the Joint POW/MIA Accounting Command-Central Identification Laboratory. These sequences are generated from degraded skeletal remains that are presumed to belong to U.S. service members missing from past military conflicts. In the laboratory, it is possible to control for contamination of remains; however, in the field, it can be difficult to prevent modern DNA from being transferred to skeletal elements and being carried forward through the analysis process. Four such cases are described here along with the controls in place in the laboratory to eliminate the possibility of the exogenous DNA being reported as authentic. In each case, the controls implemented by the laboratories prevented the false reporting of contaminant exogenous DNA from remains that were either faunal or human, but lacked endogenous DNA. Abstract published by arrangement with John Wiley & Sons.