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High-resolution melt analysis for the detection of skin/sweat via DNA methylation

NCJ Number
306064
Journal
Electrophoresis Volume: 44 Issue: 3-4 Dated: 2022 Pages: 371-377
Author(s)
Nicole Fernandez-Tejero; Quentin Gauthier; Sohee Cho; Bruce R. McCord
Date Published
December 2022
Length
7 pages
Annotation

Since up to now, there has been no specific DNA methylation-based marker to distinguish skin cell DNA from other body fluids, the goal of this study was to develop a DNA methylation-based assay to detect and identify skin cells collected at forensic crime scenes for use in DNA typing.

Abstract

Since the goal of this study was to develop a DNA methylation-based assay to detect and identify skin cells collected at forensic crime scenes for use in DNA typing, the authors utilized a DNA methylation chip array-based genome-wide association study to identify skin-specific DNA methylation markers. DNA obtained from skin along with other body fluids, such as semen, saliva, blood, and vaginal epithelia, were tested using five genes that were identified as sites for potential new epigenetic skin markers. Samples were collected, bisulfite converted, and subjected to real-time polymerase chain reaction (PCR) with high-resolution melt analysis. In our studies, when using WDR11, PON2, and NHSL1 assays with bisulfite-modified PCR, skin/sweat amplicons melted at lower temperatures compared to blood, saliva, semen, and vaginal epithelia. One-way analysis of variance demonstrates that these three skin/sweat markers are significantly different when compared with other body fluids (p < 0.05). These results demonstrate that high-resolution melt analysis is a promising technology to detect and identify skin/sweat DNA from other body fluids. (Published abstract provided)