I-DNASE21 System: Development and SWGDAM Validation of a New STR 21-Plex Reaction

• NCJ Number: 246736
• Journal: Forensic Science International: Genetics Volume: 8 Issue: 1 Dated: January 2014 Pages: 10-19
• Author(s): Jose M. Aznar; David Celorrio; Adrian Odriozola; Stephan Köhnemann; María L. Bravo; Juan J. Builes; Heidi Pfeiffer; René J. Herrera; Marian M. de Pancorbo
• Date Published: January 2014
• Length: 10 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction.

Abstract

I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System CODIS, Interpol Standard Set of Loci ISSL, Extended European Standard Set ESS-Extended, UK National Criminal Intelligence DNA Database NDNAD and German Core loci GCL. This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods SWGDAM. The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases.