NCJ Number
246863
Journal
Journal of Forensic Sciences Volume: 59 Issue: 3 Dated: May 2014 Pages: 779-784
Date Published
May 2014
Length
6 pages
Annotation
In order to devise a better forensic test for diatoms, the DNA binding ability of the diatom frustule constructing by silica, in the presence of chaotropic ions were utilized.
Abstract
In order to devise a better forensic test for diatoms, the DNA binding ability of the diatom frustule constructing by silica, in the presence of chaotropic ions were utilized. It was proved that the diatoms were able to be captured via &DNA using silica-coated magnetic beads Mag beads, followed by isolation and purification from the Mag beads as a solid phase by substituting the chaotropic agent with ultrapure water. Five cases of drowning, three in freshwater and two in seawater, were applied to the present method and similar results as the usual diatom test were obtained. Specimens of lung and other organs were rendered clearly visible, with elimination of foreign impurities. The present method appears applicable for detection of diatoms indirectly using PCR amplification of bound DNA or directly staining of the DNA. Abstract published by arrangement with Wiley.