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Korean Population Genetic Data and Concordance for the PowerPlex ESX 17, AmpFlSTR Identifiler, and PowerPlex 16 Systems

NCJ Number
Forensic Science International: Genetics Volume: 7 Issue: 3 Dated: May 2013 Pages: e47-e51
Seung Beom Hong; Soon Hee Kim; Ki Cheol Kim; Moon Hee Park; Jeung Yoon Lee; Joon Myong Song; Myun Soo Han; Wook Kim
Date Published
May 2013
5 pages
In case of paternity or maternity investigations with short tandem repeat (STR) analysis, deficient cases, missing person, or mutations are encountered and common STRs cannot provide sufficient forensic parameters. Thus, it is recommended that additional STRs are needed to complement conventional analysis for more reliable forensic information.
Researchers analyzed variation of 23 STRs contained in the new PowerPlex ESX 17 kit (Promega) and two conventional kits of the AmpFlSTR Identifiler (Applied Biosystems) and PowerPlex 16 systems (Promega) in 452 randomly chosen individuals from Korea to provide an expanded and reliable Korean database. Allele frequencies and forensic parameters were used to evaluate suitability and robustness of the new kit for forensic genetic analysis as well as in concordance studies. The combined probability of match for the 16 loci in the PowerPlex ESX 17 system was 2.76 10−20. One genotyping discrepancy due to a null allele was observed at the D18S51 locus (the concordant rate = 99.99 percent), showing a primer-binding site mutation in the sequence of the locus (G-to-A substitution at position 146 of Genbank accession number JX018211). Thus, the new kit is a valuable forensic tool and is suitable to extend the Korean population genetic data obtained with well-established polymerase chain reaction multiplex-kits of the AmpFlSTR Identifiler and PowerPlex 16 systems.(Published Abstract)