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Quantitative Method for Simultaneous Determination of 5-Methoxy-N,N-Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry

NCJ Number
Journal of Forensic Sciences Volume: 56 Issue: 4 Dated: July 2011 Pages: 1044-1048
Ming J. Jin, Ph.D.; Changbae Jin, Ph.D.; Jin Y. Kim, Ph.D.; Moon K. In, Ph.D.; Oh S. Kwon, Ph.D.; Hye H. Yoo, Ph.D.
Date Published
July 2011
5 pages
This article discusses the detection of 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) used as recreationally psychedelic drug.
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography-tandem mass spectrometric method for 5-MeO-DIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C(18) column (50 x 2.0 mm, 5 um) for chromatographic separation. Mobile phases consisted of methanol, water, and 1percent formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01-10 ug/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra- and interday accuracies and precisions met the criteria (less than 15 percent). The developed method was successfully applied to the drug-treated rat urine. (Published Abstract)