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Recovery of Whole Mitochondrial Genome From Compromised Samples via Multiplex PCR and Massively Parallel Sequencing

NCJ Number
Future Science Oa Volume: 4 Issue: 9 Dated: 2018
Maureen P. Hickman; Kelly S. Grisdale; Brittania J Bintz; Erin S. Burnside; Erin K. Henson; Jack ballantyne; Mark R. Wilson
Date Published
12 pages
This article reports on a project that developed a multiplex PCR method that amplifies the complete mitochondrial genome in a range of forensically relevant samples, including single cells, cremated remains, bone, maggot, and hairs isolated from dust bunnies.

In forensic casework, compromised samples often have limited or degraded nuclear DNA, rendering mitochondrial DNA a more feasible option for forensic DNA analyses. The emergence of massively parallel sequencing (MPS) has enabled the recovery of extensive sequence information from low quantities of DNA. In the current project, MPS yields completed or nearly completed mitochondrial genome coverage for all samples. To confirm concordance between sample types and between sequencing platforms, the project compared sequencing results from hair and buccal swabs from two references. Low initial DNA input into the multiplex PCR allowed for conservation of precious DNA, while MPS maximized recovery of genetic information. (publisher abstract modified)