NCJ Number
248140
Journal
Forensic Science International Genetics Volume: 12 Dated: September 2014 Pages: 38-41
Date Published
September 2014
Length
4 pages
Annotation
Given that the implementation of second- generation-system (SGS) in forensic genetics requires a complete re-evaluation of the current STR frequencies database due to the identification of more alleles, this project reports an STR-sequence database with sequence and allele frequencies of two compounds (D351358 and D125391) and one complex STR D21S11) [13-17], using 197 unrelated residents of Denmark.
Abstract
The project used nomenclature for sequenced STR alleles that may be used in future studies and applications of STR profiling. The use of SGS instead of the traditional PCR-CE fragment analysis for D3S1358, D12S391 resulted in the identification of 2.75 times more alleles, higher typical paternity indices and improved power of discrimination. The additional information that is obtained with SCS compared to PCR-CE is only one of the advantages of SGS. Combining genotyping of STRs and SNPs in the same assay will have a large impact on forensic genetics, and it will give research and case officers important new information. The provider of the platform used in this work (Roche) has recently announced that production of the 454 sequencers will be terminated in 2015. Thus, the 454 GS junior platform is not likely to be the future forensic genetic platform; however, two other major providers of SGS data analysis will be a vital point for the introduction of SGS into forensic genetics, because the amount of sequence data is so large that the user will be unable to analyze the profile in detail, having to rely on the software solutions. Several bioinformatics tools are already under development; however, a close collaboration between companies and the forensic community will be essential for the development of better and fully automated SGS software. 2 tables and 18 references