Use of APTIMA Combo 2: The Experience of a Child Advocacy Center

• NCJ Number: 245127
• Journal: Journal of Child Sexual Abuse Volume: 22 Issue: 3 Dated: 2013 Pages: 297-311
• Author(s): M. Ranee Leder; Amy L. Leber; Mario J. Marcon; Philip V. Scribano
• Date Published: 2013
• Length: 15 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

This study discusses nucleic acid amplification testing for chlamydia and gonorrhea in sexually abused girls.

Abstract

The Centers for Disease Control and Prevention recommends nucleic acid amplification testing for chlamydia and gonorrhea in sexually abused girls. No studies describe performance of APTIMA Combo 2 Assay with second target confirmation on the same testing platform. This nucleic acid amplification testing is evaluated within a large child advocacy center. Girls 3 to 18 years, 35% of whom reported consensual sexual activity, were prospectively tested by APTIMA Combo 2 on urine/vaginal swabs and by vaginal culture. A case of infection was defined as positive culture or positive urine or vaginal swab nucleic acid amplification testing with second target confirmation. Sensitivity of APTIMA Combo 2 on urine was found to be superior to vaginal culture and comparable to APTIMA Combo 2 on vaginal swabs for both infections. APTIMA Combo 2 on urine is less invasive, and its use may be preferred in this traumatized population.