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Validation of SRY Marker for Forensic Casework Analysis

NCJ Number
Journal of Forensic Sciences Volume: 54 Issue: 3 Dated: May 2009 Pages: 551-555
Vanja Kastelic B.S.; Bruce Budowle Ph.D.; Katja Drobnic Ph.D.
Date Published
May 2009
5 pages
This study performed some validation studies on the SRY gene (gender determination) marker for use in forensic cases, with attention to repeatability, sensitivity, gender specificity, and mixture samples.
The validation studies conducted support the sensitivity and reliability of the SRY male gender marker developed by Drobnic, and it can be used in concert with commercially available human single tandem repeat (STR) identification kits in order to type DNA derived from forensic samples. The robustness and sensitivity of the SRY assay in mixed samples was demonstrated at mixture ratios of 1:16 with a total of 93.7 pg male DNA compared with the AMELY allele assay. Mixtures with low amounts of male DNA amidst high concentrations of female DNA can be typed with the SRY male gender marker assay. The SRY markers as a singleplex or included in multiplex kits can be used as an adjunct to standard gender typing. Future studies should include large population scale SRY analyses in order to determine whether drop-out is sufficiently low for forensic gender typing. The study was conducted with 115 unrelated male Slovenians. Quantification of DNA was conducted with the Quantifiler Human DNA Quantification Kit with mL of DNA extract on the ABU Prism 7000 Sequence Detection system. Amplification of DNA was performed using the SRY primers under AmpFISTR SGM Plus manufacturer’s recommendations. A singleplex DNA amplification was conducted in a total volume of 25 mL containing 5 U/mL AmpliTaq Gold Polymerase, 0.2 mm forward SRY primer, 0.24 mm reverse SRY primer, and 10.0 mL 10x Gold STR buffer in a Perkin-Elmer 9600 thermal cycler. A singleplex DNA amplification was used in all studies except for the mixture study. Mixture studies were conducted as multiplex amplification. 5 figures and 20 references