Validation of Two Canine STR Multiplex-Assays Following the ISFG Recommendations for Non-Human DNA Analysis

• NCJ Number: 246745
• Journal: Forensic Science International: Genetics Volume: 8 Issue: 1 Dated: January 2014 Pages: 90-100
• Author(s): B. Berger; C. Berger; W. Hecht; A. Hellmann; U. Rohleder; U. Schleenbecker; W. Parson
• Date Published: January 2014
• Length: 11 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

To gain general acceptance forensic DNA testing in animals needs to improve standardization of analysis methods and data interpretation.

Abstract

To gain general acceptance forensic DNA testing in animals needs to improve standardization of analysis methods and data interpretation. Recently, the International Society of Forensic Genetics ISFG took particular care of this topic by publishing recommendations for forensic non-human DNA analysis following the successful example of human DNA analysis in order to provide a basis for harmonization of the still existing inter-laboratory variability. By following these recommendations we demonstrate the performance of two short tandem repeat STR multiplexes for forensic identity testing of canine biological material. Thirteen STRs and two sex-specific markers were selected and validated according to the ISFG guidelines. Population genetic parameters were calculated based on 295 dog samples collected in Austria 124 and Germany 171. A repeat-based nomenclature of the mainly tetrameric STRs and corresponding allelic ladders are presented. All 146 different alleles included in the ladders were sequenced for correct allele calling. Additionally, a canine cell line DH82-D3167 was evaluated as standard reference material.