U.S. flag

An official website of the United States government, Department of Justice.

Characterization of the Interaction Between Rfa1 and Rad24 in Saccharomyces cerevisiae

NCJ Number
249432
Journal
PLoS ONE Volume: 10 Issue: 2 Dated: February 2015
Date Published
February 2015
Length
0 pages
Annotation

In order to gain a better understanding of how RPA functions in DNA replication, repair, and cell cycle regulation and to identify other potential functions of RPA, this study performed a yeast two-hybrid screen using the yeast 70 kDa subunit, Replication Factor A1 (Rfa1), as a bait protein.

Abstract

Analysis of 136 interaction candidates resulted in the identification of 37 potential interacting partners, including the cell cycle regulatory protein and DNA damage clamp loader Rad24. The Rfa1-Rad24 interaction is not dependent on ssDNA binding; however, this interaction appears affected by DNA damage. The regions of both Rfa1 and Rad24, important for this interaction, were identified; and the region of Rad24 identified is distinct from the region reported to be important for its interaction with Rfc2 5. This suggests that Rad24-Rfc2-5 (Rad24-RFC) recruitment to DNA damage substrates by RPA occurs, at least partially, through an interaction between the N terminus of Rfa1 and the C terminus of Rad24. The predicted structure and location of the Rad24 C-terminus is consistent with a model in which RPA interacts with a damage substrate, loads Rad24-RFC at the 5' junction, and then releases the Rad24-RFC complex to allow for proper loading and function of the DNA damage clamp. (Publisher abstract modified)

Date Published: February 1, 2015