Since the United States and many other countries worldwide are currently experiencing a public health crisis due to the abuse of heroin and illicitly manufactured fentanyl, the current project developed a liquid chromatography tandem mass spectrometry (LC–MS-MS)-based method for the detection of morphine, fentanyl, and their metabolites, including morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G), normorphine, norfentanyl and deuterated internal standards in limited sample volumes with the limit of detection of 5.0/0.5 ng/mL (morphine, M3G, M6G, normorphine/fentanyl, norfentanyl).
The inter-assay precision (percent CV) was less than 12 percent for all assays, and the inter-assay bias (percentage) was less than 5 percent. The ruggedness of the method, dilution effect, and carryover were also investigated as part of the study. The simultaneous quantification of morphine, fentanyl, and its metabolites with this simple and time- and cost-efficient method could be successfully applied to samples taken for pharmacokinetic evaluation (antemortem and postmortem) after a single dose of morphine or co-administration of morphine with other drugs (e.g., fentanyl) in rats. (publisher abstract modified)
Downloads
Similar Publications
- Targeting the "Absence" in a Desistance Framework: Balancing Risk and Rehabilitation in Mandated Criminal Background Check Employment Decisions
- The Influence of Social Surroundings on Juvenile and Criminal Justice Involvement of 17-Year-Olds Transitioning from Foster Care to Adulthood: A Longitudinal and Life-Course Approach
- Biomechanical assessment of fracture risk in young children with healthy bone and osteogenesis imperfecta.