Colorimetry with microfluidic devices has been proven to be an advantageous method for in situ analyses where limited resources and rapid response for untrained users are desired. Image analysis using a small camera or cell phone can be easily incorporated for an objective readout, eliminating variations from normal differences in color perception and environmental factors during analysis. The image analysis using the parameter hue, for example, has been utilized as a highly effective, objective analysis method that correlates with the psychological way color is perceived. In the current project, tinting is invoked by dyeing the reagent substrate, colored printing the device, or colored lighting during image capture, and is shown to shift the background color of the reaction detection area. Hydrogen peroxide, a constituent of peroxide-based explosives, is associated with a monochromatic color change upon reaction, and this is used to demonstrate the effectiveness of the tinting methods in improving the limit of detection from an undetectable color change to 0.1 mg mL-1. (publisher abstract modified)
Downloads
Similar Publications
- The Role of Simulated Data in Making the Best Predictions (from the 87th Annual Meeting of the American Association of Physical Anthropologists - 2018)
- Evidence Management Steering Committee Report: Opportunities to Strengthen Evidence Management Processes
- Principles of Fluorogenic Reagent Design for Forensics. Recent Progress Towards New Reagents to Develop Fingerprints in Blood and on Variable Surfaces