DNA testing for food authentication and quality control requires sensitive species-specific quantification of nuclear DNA from complex and unknown biological sources. The multiplex assay developed in the current project is able to accurately detect very low quantities of species-specific DNA from single or multispecies sample mixtures; its minimum effective quantification range is 5 to 50 pg of starting DNA material. In addition to its use in food fraudulence cases, the project validated the assay using simulated forensic sample conditions to demonstrate its utility in forensic investigations. Despite treatment with potent inhibitors such as hematin and humic acid, and degradation of template DNA by DNase, the assay was still able to robustly detect and quantify DNA from each of the three poultry species in mixed samples. The efficient species determination and accurate DNA quantification will help reduce fraudulent food labeling and facilitate downstream DNA analysis for genetic identification and traceability. (Publisher abstract modified)
Downloads
Similar Publications
- Just Improving Sexual Assault Kit Testing Workflows
- Using Citizen Notification To Interrupt Near-Repeat Residential Burglary Patterns: the Micro-Level Near-Repeat Experiment
- Using Clonal Massively Parallel Sequencing to Characterize Heteroplasmy in the mtDNA of Human Head Hair, Pubic Hair, and Buccal Samples