This article assesses the RapidINTEL Plus Sample cartridge across seven RapidHIT instruments, demonstrating little variation in sensitivity among the instruments, based on heatmap consistencies.
Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages. (Published Abstract Provided)
Downloads
Similar Publications
- Transfer, Persistence and DNA Source Attribution of Trace Biological Material in Digital Penetration Assault Cases
- Development of baseline survey of random presence of glass and paint for the interpretation of evidence in the US courts
- Technical Note: A novel method for simultaneous recovery of DNA, RNA, and proteins from trace biological samples for forensic application