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Flow cytometry analysis of epithelial cell populations from touch samples using the BD Influx flow cytometry platform

NCJ Number
310924
Journal
F1000Research Volume: 5 Issue: 390 Dated: 2016
Author(s)
Ye Jin Kwan; Cristina E. Stanciu; M. Katherine Philpott; Christopher J. Ehrhardt
Date Published
2016
Abstract

‘Touch’ or trace cell mixtures submitted as evidence are a significant problem for forensic laboratories as they can render resulting genetic profiles difficult or even impossible to interpret. Optical signatures that distinguish epidermal cell populations from different contributors could facilitate the physical separation of mixture components prior to genetic analysis, and potentially the downstream production of single source profiles and/or simplified mixtures.  For this dataset, optical properties including forwards scatter (FSC), side scatter (SSC), and fluorescence emissions in the Allophycocyanin (APC) channel were measured in epithelial cell populations from touch samples collected from several different contributors on multiple days to assess inter- and intra-contributor variability.

(Publisher abstract provided.)