Forensic Identification of Indian Snakeroot Rauvolfia serpentina Benth. ex Kurz Using DNA Barcoding

• NCJ Number: 246122
• Journal: Journal of Forensic Sciences Volume: 58 Issue: 3 Dated: May 2013 Pages: 822-830
• Author(s): Marcel C. M. Eurlings, B.AS.; Frederic Lens, Ph.D.; Csilla Pakusza, B.Sc.; Tamara Peelen, Ph.D.; Jan J. Wieringa, Ph.D.; Barbara Gravendeel, Ph.D.
• Date Published: May 2013
• Length: 9 pages
• Publication Format: Article
• Publication Type: Report (Technical Assistance); Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

This research examined species-specific DNA polymorphisms for Indian snakeroot (Rauvolfia serpentina).

Abstract

Indian snakeroot (Rauvolfia serpentina) is a valuable forest product, root extracts of which are used as an antihypertensive drug. Increasing demand led to overharvesting in the wild. Control of international trade is hampered by the inability to identify root samples to the species level. Researchers therefore evaluated the potential of molecular identification by searching for species-specific DNA polymorphisms. The researchers found two species-specific indels in the rps16 intron region for R. serpentina. The DNA barcoding method was tested for its specificity, reproducibility, sensitivity and stability. Researchers included samples of various tissues and ages, which had been treated differently for preservation. DNA extractions were tested in a range of amplification settings and dilutions. Species-specific rps16 intron sequences were obtained from 79 herbarium accessions and one confiscated root, encompassing 39 different species. The results demonstrate that molecular analysis provides new perspectives for forensic identification of Indian snakeroot. Abstract published by arrangement with John Wiley & Sons.