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Optimization of novel loop-mediated isothermal amplification with colorimetric image analysis for forensic body fluid identification

NCJ Number
304514
Journal
Journal of Forensic Sciences Volume: 66 Issue: 3 Dated: 2021 Pages: 1033-1041
Author(s)
T. Layne; et al
Date Published
2021
Length
9 pages
Annotation

This study presents preliminary data on a loop-mediated isothermal amplification (LAMP) method that rapidly identifies body fluids, including venous blood, semen, and saliva, based on colorimetric response and image analysis.

Abstract

Accurate presumptive and confirmatory test use for forensic body fluid identification is essential for gaining contextual information for crime scene investigators. Loop-mediated isothermal amplification (LAMP) is an ideal method for forensic body fluid identification because it is highly specific and generates multi-sized amplicon DNA, and successful amplification results can be read out colorimetrically. The LAMP method is designed for easy implementation into forensic casework protocols with minimal disruption to DNA analysis. LAMP naturally increases target specificity due to the use of multiple primers for one target and mRNA targets were used for tissue and human specificity. With colorimetric detection as an inherent part of LAMP, samples that are positive or negative for any of the body fluids are readily identified by image capture and analysis, thus eliminating subjectivity. Results show by using the 3D-printed imaging system specific color ranges can be set for easy determination of body fluids. The resulting color change can be seen in <30 min using a universal temperature and primer concentration for all body fluids. This simple method and imaging system allow for minimal hands-on time with objective image analysis and presents a pathway for creating a new potential method for forensic body fluid identification. (Publisher Abstract)