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SERS-based plasmonic nanobiosensing in single living cells

NCJ Number
Analytical and Bioanalytical Chemistry Volume: 393 Dated: 2009 Pages: 1135–1141
Jonathan P. Scaffidi; Molly K. Gregas; Victoria Seewaldt ; Tuan Vo-Dinh
Date Published
7 pages

This paper describes the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection.


The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized “normal” human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level. (Publisher Abstract)