The accurate identification of body fluids from crime scenes can aid in discriminating between offender and victim fluids found at the scene. Researchers bisulfite-modified and amplified DNA samples from blood, saliva, semen, and vaginal epithelia, using primers for PFN3A. Using pyrosequencing, they showed that vaginal epithelia present distinct methylation levels when compared to other body fluids. Mixtures of different body fluids present methylation values that correlate with single-source body fluid samples, and the primers for PFN3A are specific for primates. This project successfully demonstrated that the analysis of methylation in the PFN3A locus can be used for vaginal epithelia discrimination in forensic samples. (Publisher abstract modified)
Downloads
Similar Publications
- Visualizing Deep Similarity Networks
- A Systematic Study of Liquid Chromatography in Search of the Best Separation of Cannabinoids for Potency Testing of Hemp-Based Products Using Diode Array Detector and Electrospray Ionization Mass Spectrometry
- EVALUATING THE SUCCESS OF A KINETIC MODEL TO PREDICT CHROMATOGRAMS OF IGNITABLE LIQUIDS UNDER DIFFERENT EVAPORATION MODES AND IN THE PRESENCE OF PASSIVE-HEADSPACE EXTRACTION