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A method to generate capture baits for targeted sequencing

NCJ Number
307120
Journal
Nucleic Acids Research Dated: 2013
Date Published
2023
Length
11 pages
Annotation

In this paper, the authors demonstrate the use of the CNER method for targeted genotyping by producing a set of CNER probes to capture 23771 SNPs in the horse genome, using ten ancient horse DNA libraries of varying endogenous DNA content and DNA degradation levels, and show that the CNERs effectively perform target enrichment even in highly degraded ancient samples comparably.

Abstract

Hybridization capture approaches allow targeted high-throughput sequencing analysis at reduced costs compared to shotgun sequencing. Hybridization capture is particularly useful in analyses of genomic data from ancient, environmental, and forensic samples, where target content is low, DNA is fragmented and multiplex PCR or other targeted approaches often fail. Here, the authors describe a DNA bait synthesis approach for hybridization capture that they call Circular Nucleic acid Enrichment Reagent, or CNER (pronounced ‘snare’). The CNER method uses rolling-circle amplification followed by restriction digestion to discretize microgram quantities of hybridization probes. The authors demonstrate the utility of the CNER method by generating probes for a panel of 23 771 known sites of single nucleotide polymorphism in the horse genome. Using these probes, they capture and sequence from a panel of ten ancient horse DNA libraries, comparing CNER capture efficiency to a commercially available approach. With about one million read pairs per sample, CNERs captured more targets (90.5% versus 66.5%) at greater mean depth than an alternative commercial approach. (Publisher Abstract Provided)

Date Published: January 1, 2023