Plant leaves were analyzed in their native form by DART-MS without the need for any sample preparation steps. This furnished chemical fingerprints characteristics of each species. In the same experiment, in-source collision-induced dissociation was used to identify biomarkers. Biomarker presence was also independently confirmed by GC-MS. Chemometric processing of DART-MS profiles was performed by kernel discriminant analysis (KDA) and soft independent modeling of class analogy (SIMCA) to classify the fingerprints according to species. The approach was successful despite the occurrence of diurnal cycle and plant-age related chemical profile variations within species. In a single rapid experiment, the presence of essential oil biomarkers such as 3-carene, a-pinene, B-theorem, B-caryophyllene, camphoe, and boneol could be confirmed. The method was applied to rapid identification and differentiation of Salvia apiana S. dominica, S. elegans, S. officinalis, S. farinacea, and S. patens. The study concludes that species-level identification of Salvia plant material could be accomplished by chemometric processing of DART-HRMS-derived chemical profiles of both fresh and dried Salvia material. (Publisher abstract modified)
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