NCJ Number
240067
Journal
Forensic Science International: Genetics Volume: 6 Issue: 5 Dated: September 2012 Pages: 588-593
Date Published
September 2012
Length
6 pages
Annotation
The script presented in this paper, was designed to automatically generate DNA consensus profiles from at least two PCR replicates using the Bracket method.
Abstract
The authors of this article presents an automated method to generate DNA profiles from replicate PCRs by combining advantages of the composite and consensus method by a system of brackets in which an allelic balance threshold is used as a variable to separate DNA-profiles of major from minor donors. Through the analysis of artificial low (125 pg) and high (250 pg) template three-person mixtures with low (1:1.5:3) and high (1:5:10) donor ratios, the authors demonstrate the usefulness of a tool to determine the optimal allelic balance threshold within a locus. The automated extraction of dominant profiles saves considerable amounts of time when producing composite-consensus profiles. Drop-in/drop-out rates are produced and a comparison is made with an alternative open source script to evaluate the dominant profiles generated. By introducing this script into the forensic community the authors hope to increase awareness of much needed collaborative efforts with bioinformaticians and statisticians to develop forensic open source software scripts. (Published Abstract)