NCJ Number
132869
Date Published
1990
Length
381 pages
Annotation
This text discusses the principles and basic methodology for DNA analysis to identify tissues and individuals.
Abstract
The introduction contains the definition, history, and applications of DNA fingerprinting as well as a case for its use. This is followed by a description of genetic principles pertinent to DNA analysis. A discussion of laboratory organization for DNA analysis precedes a review of procedures for the management of specimens. DNA amplification is also addressed, and various DNA analysis techniques are explained. Other chapters cover probes, allele mutations, and restriction enzymes; probability and statistical analysis; quality control; and legal and ethical considerations. Descriptions of specific applications of the principles and laboratory methods discussed encompass forensic analysis, paternity testing, medical diagnostics, animal and plant sciences, and wildlife poaching. Appendixes contain guidelines for a quality assurance program for DNA restriction fragment length polymorphism analysis; a theoretical model for a combined DNA index system; and a list of suppliers of DNA analysis equipment, products, or services. Glossary, abbreviations and symbols, and name and subject indexes