Recovery of Genomic DNA From Archived PCR Product Mixes for Subsequent Multiplex Amplification and Typing of Additional Loci: Forensic Significance for Older Unsolved Criminal Cases

• NCJ Number: 196324
• Journal: Journal of Forensic Sciences Volume: 47 Issue: 4 Dated: July 2002 Pages: 786-796
• Author(s): Kylie L. Patchett M.Sc.; Ken J. Cox B.Sc.; Dennis M. Burns Ph.D.
• Date Published: July 2002
• Length: 11 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

This article investigates a method for genomic DNA recovery from different types of PCR product mixes suitable for multiplex amplification and typing using the Profiler Plus STR typing system.

Abstract

Application of this method is significant in cases where the original DNA samples have been exhausted by repeated typing analyses in an effort to maximize their evidentiary value. Such cases typically involved samples analyzed using DNA typing systems of the time, which gave a markedly lower power of discrimination, either alone or in combination. An effective method for recovering genomic DNA from triplex amplification mixes, suitable for reproducible achievement of the complete Profiler Plus profile, involved use of Amicon Micron-100 microconcentrators. For some older casework investigations, biological material collected from crime scenes and the corresponding DNA extracts might have become exhausted. In such instances, the only material remaining was the unused portions of amplification product mixes made using one or more of the PCR-based typing systems, which have been superseded by Profiler Plus. Figures, references