Selective Quantification of Human DNA by Real-Time PCR of FOXP2

• NCJ Number: 239562
• Journal: Forensic Science International: Genetics Volume: 6 Issue: 4 Dated: July 2012 Pages: 447-451
• Author(s): Mikiko Soejima; Kenichi Hiroshige; Joji Yoshimoto; Yoshiro Koda
• Date Published: July 2012
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: Ireland

Annotation

This article discusses a simple quantitative PCR procedure with high specificity and sensitivity using TaqMan probes targeting the FOXP2 sequence.

Abstract

The authors established a simple quantitative PCR procedure with high specificity and sensitivity using TaqMan probes targeting the FOXP2 sequence. This assay distinguished human and nonhuman, including primates, samples with the exception of mouse, turtle, lizard, and fishes. However, the specific amplification of mouse, lizard, and turtle fragments of FOXP2 could be confirmed by electrophoresis after real-time PCR. Because the C(T) values obtained for human DNA were not affected by contaminating animal DNA at concentrations up to 30 times that of human DNA, we were able to estimate the concentration of human DNA in mixed specimens. This assay provides a reliable and useful method for routine quantification of human-specific DNA in forensic practice. (Published Abstract)