NCJ Number
210791
Journal
Journal of Forensic Sciences Volume: 50 Issue: 4 Dated: July 2005 Pages: 975-977
Date Published
July 2005
Length
3 pages
Annotation
This paper reports on the amplification of the multi-copy tetranucleotide Y-chromosome short tandem repeat (Y-STR) marker DYS464 for 679 male U.S. population samples as part of a multiplex Y-STR assay.
Abstract
The samples obtained included 262 Caucasians, 265 African-Americans, and 152 Hispanics. Amplification was done with the following PCR primers: VIC-CTTTGGGCTATGCCTCAGTTT and GCCATACCTGGGTAACAGAGAGAC. This set of primers target at least 4 separate regions of the Y-chromosome and can generate up to 4 distinct peaks in the size range of 242-286 bp that have an allele range of 9-20 CCTT repeats. A table presents a summary of the haplotypes observed based on peak height information with the DYS464 locus. Across the 679 samples examined, 179 different expanded types were observed, with 92 occurring only once. If the information presented in the table is collapsed to include only the peaks observed (no consideration of the relative peak heights), then 113 conservative types are evident. In 13 individuals, there were apparently more than 4 copies of DYS464 present, based on the allele peak heights. In addition, 10 samples appeared to have a deletion of 1 of the copies. This phenomenon of Y-chromosomal duplication and deletion has been observed with other Y-STR markers used in human identity testing. The authors advise that DYS464 is a valuable, highly polymorphic marker that can be useful in paternity testing and genetic genealogy applications. 1 table and 8 references