SNP-microarrays Can Accurately Identify the Presence of an Individual in Complex Forensic DNA Mixtures

• NCJ Number: 249336
• Journal: Forensic Science International - Genetics Volume: 16 Dated: May 2015 Pages: 208-215
• Date Published: May 2015
• Author(s): Lev Voskoboinik; Sheri B. Ayers; Aaron K. Lefebvre; Ariel Darvasi
• Length: 8 pages
• Grant Number(s): 2011-DN-BX-K555
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical); Report (Study/Research); Report (Grant Sponsored)

Annotation

This study evaluated the practical usefulness of currently available "off-the-shelf" SNP microarrays for identifying the presence of an individual in a mixture of several contributors in DNA samples from a mass disaster.

Abstract

The study found that a set of 3000 SNPs specifically selected for this purpose can accurately identify the presence of an individual in complex DNA mixtures of various compositions; for example, individuals contributing as little as 5 percent to a complex DNA mixture can be robustly identified even when the starting DNA amount is as little as 5.0 ng and had undergone whole-genome amplification (WGA) prior to SNP analysis. The work presented in this study represents proof-of-principle that the authors previously proposed approach can work with real "forensic-type" samples. Furthermore, in the absence of a low-density focused forensic SNP microarray, the use of standard, currently available high-density SNP microarrays can be similarly used and even increase statistical power due to the larger amount of available information. (Publisher abstract modified)