NCJ Number
146643
Journal
Journal of Forensic Sciences Volume: 39 Issue: 1 Dated: (January 1994) Pages: 52-63
Date Published
1994
Length
12 pages
Annotation
Polymerase chain reaction (PCR) analysis of the variable number of tandem repeat (VNTR) locus D17S30, a minisatellite region located on chromosome 17, was assessed for its potential use in forensic identification.
Abstract
Whole blood samples were collected by venipuncture under sterile conditions in 10 mL ethylenediaminetetraacetic acid and stored at 40 degrees C until extraction. Buccal cell samples were collected by rubbing the cotton tip of a sterile swab on the inside of both cheeks. DNA was extracted from both whole blood and oral swabs. PCR conditions were varied in an effort to improve allele amplification at the D17S30 locus. Such variations included the use of denaturants, formamide, and dimethylsulphoxide to overcome any incomplete denaturation of template strands. Lowering the annealing temperature during the PCR cycle enhanced the amplification of a larger fragment, but this was not related to the D17S30 locus. It appeared that the genome structure of some individuals rendered PCR amplification inefficient at this locus. 17 references and 6 figures