NCJ Number
19931
Journal
Journal of the Forensic Science Society Volume: 14 Issue: 2 Dated: (APRIL 1974) Pages: 117-121
Date Published
1974
Length
5 pages
Annotation
A METHOD OF DETERMINING THE NATURE OF ANTIBODIES IN HUMANS EVEN THOUGH MANY YEARS MAY HAVE ELAPSED SINCE THE SUBJECT ORIGINIALLY CAME IN CONTACT WITH THE ANTIGEN.
Abstract
ANTIBODIES OT SPECIFIC PARASITE MAY BE READILY DETECTED IN ELUATES OF DRIED BLOODSTAINS. THE METHOD IS BASED UPON THE REACTION OF A SERUM ANTIBODY WITH AN ANTIGEN ON A MICROSCOPE SLIDE. THE PRESENCE OF A POSITIVE REACTION IS VISUALIZED BY FLORESCENCE MICROSCOPY AFTER REACTING THE ANTIGEN-ANTIBODY COMPLEX WITH FLORESCEIN-LABELLED ANTI-HUMAN IMMUNOGLOBULIN. THE FREQUENCY OF ANTIBODIES TO FIVE ANTIGENS IN A RANDOM SAMPLE OF BLOODSTAINS HAS BEEN OBTAINED AND THE DISCRIMINATING POWER OF THESE FIVE ALONE IS FOUND TO BE BETTER THAN THAT ACHIEVED BY THE ABO BLOOD GROUPING SYSTEM. OTHER APPLICATIONS OF THE TECHNIQUE IN FORENSIC BIOLOGY ARE BRIEFLY DISCUSSED. (AUTHOR ABSTRACT)